Abstract
Protein arginine methyltransferases (PRMTs) are involved in many cellular processes via the arginine methylation of histone or non-histone proteins. We examined the expression patterns of prmt4, prmt7, and prmt9 during embryogenesis in Xenopus using whole-mount in situ hybridization and quantitative reverse transcription polymerase chain reaction (RT-PCR). Xenopus prmt4 and prmt7 were expressed in the neural crest, brain, and spinal cord, and also detected in the eye, branchial arches, and heart at the tailbud stage. Specific prmt9 signals were not detected in Xenopus embryos until the late tailbud stage when weak expression was observed in the branchial arches. Quantitative RT-PCR indicated that the expressions of prmt4 and prmt7 were up-regulated during the neurula stage, whereas prmt9 maintained its low expression until the late tailbud stage, consistent with the whole-mount in situ hybridization results. Thus, the developmental expression patterns of these three prmt genes in Xenopus embryos provide a basis for further functional study of such genes.
Highlights
Protein arginine methyltransferases (PRMTs) are involved in many cellular processes via the arginine methylation of histone or non-histone proteins
Limited studies have shown that PRMT4 and PRMT7 are involved in myogenesis (Batut et al, 2011; Blanc et al, 2016; Mallappa et al, 2011)
Whole-mount in situ hybridization could not detect specific signals of prmt9 in Xenopus embryos at almost every stage, though a weak signal was observed in the branchial arches at the late tailbud stage
Summary
Protein arginine methyltransferases (PRMTs) are involved in many cellular processes via the arginine methylation of histone or non-histone proteins. We performed whole-mount in situ hybridization to examine the spatial expression of prmt genes in Xenopus embryos. At the early neurula stage, the prmt4 signal was enriched in the anterior region of the neural plate as well as the posterior region around the blastopore (Figure 1B). Prmt4 was detected throughout the central nervous system, including the brain and spinal cord (Figure 1G, H).
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