Developmental expression and subcellular localization of wallaby gonadotropin-releasing hormone receptor and its splice variants

Abstract

The developmental expression of gonadotropin-releasing hormone receptor (GnRH-R) and its splice variants was examined in the gonads of tammar wallaby pouch young in order to elucidate the functional role of GnRH-R in the developing testis and ovary. Wallaby GnRH-R, like eutherian GnRH-Rs, contains three exons and two introns. In the present study, the transcripts of two splice variants (GnRH-RΔ1 and GnRH-RΔ2) were cloned from the pituitary. GnRH-RΔ1 contained a 291 bp deletion from nucleotide positions 232 to 522 within exon 1. This transcript appears to be distinctive in the wallaby and has not been reported in other species. GnRH-RΔ2 contained a 220 bp deletion from nucleotide positions 523 to 742, corresponding to exon 2. We examined the subcellular localization of the wild type GnRH-R and its splice variants with confocal microscopy, showing that both the wild type receptor and the splice variants were membrane-associated molecules. The different pattern of expression of the wild type receptor and the variants transcripts found in adult and neonatal tissues suggests a specific developmental regulation of the GnRH-RΔ2 transcript. In addition, the developmental expression of the GnRH-R and GnRH-RΔ1 transcripts showed a possible association with key physiological events during gonadal development in the wallaby pouch young, suggesting that GnRH-R may be involved in the regulation of early development in the testis and ovary.