Abstract
Changes of carbohydrate content of a developing rose shoot, submitted to different day-night temperature conditions were investigated. Two successive experiments were set up in four glasshouse compartments with young rose plants (Rosa hybrida 'Frisco'). The plants were submitted to four day/night temperature regimes (19.3/12 degreesC, 18/16 degreesC, 17.5/17.5 degreesC and 16/22 degreesC) maintaining a daily mean temperature of 17.5 degreesC. The plants were grown under a photoperiod of 18 hours. The experiment started at decapitation and ended when all shoots were harvested. The light integral was 148 MJ/m(2) for the first experiment and 307 MJ/m2 for the second experiment. Carbohydrate measurements (glucose, fructose, sucrose and starch) were performed on the entire uppermost shoots, source leaves and sink tissue. Four different developmental stages of the shoot bud were analysed: stage 1 - vegetative bud, stage 4 - petal differentiation, stage 5/6 - pistil and stamen differentiation and a harvestable flower stem. The results show that both developmental stages and light intensity have a strong impact on the carbohydrate level of the shoot and source leaf while temperature regime has no direct effect.
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