Abstract
A solid state electrochemiluminescence (ECL) sensor based on Ru(bpy)32+-encapsulated silica nanoparticles (RuNP) covalently immobilised on a screen printed carbon electrode has been developed and characterised. RuNPs were synthesised using water-in-oil microemulsion method, amino groups were introduced on their surface, and they were characterised by transmission electron microscopy. Aminated RuNPs were covalently immobilised on activate screen-printed carbon electrodes to form a solid state ECL biosensor. The biosensor surfaces were characterised using electrochemistry and scanning electron microscopy, which showed that aminated nanoparticles formed dense 3D layers on the electrode surface thus allowing immobilisation of high amount of Ru(bpy)32+. The developed sensor was used for ECL detection of biogenic polyamines, namely spermine, spermidine, cadaverine and putrescine. The sensor exhibited high sensitivity and stability.
Highlights
Electrogenerated chemiluminescence or electrochemiluminescence (ECL) is an analytical technique based on light emission at the surface of an electrode from compounds that have reached the excited state via electrochemical reactions [1,2,3,4].ECL can be classified as a spectro-electrochemical method
Sensitive immunoassay detection down to sub pg/mL levels has been reported [27]. In this contribution we report on the development of solid state ECL sensors based on the immobilisation of Ru(bpy)32+ on silica nanoparticles deposited on screen printed carbon electrodes
Simultaneous detection of analytes was not attempted; in the future integration of the ECL sensor with a separation method could be envisaged. In this contribution we have described fabrication of a solid state ECL sensor, based on covalent immobilization of Ru(bpy)32+-encapsulated silica nanoparticles on screen printed carbon electrode
Summary
Electrogenerated chemiluminescence or electrochemiluminescence (ECL) is an analytical technique based on light emission at the surface of an electrode from compounds that have reached the excited state via electrochemical reactions [1,2,3,4].ECL can be classified as a spectro-electrochemical method. Electrogenerated chemiluminescence or electrochemiluminescence (ECL) is an analytical technique based on light emission at the surface of an electrode from compounds that have reached the excited state via electrochemical reactions [1,2,3,4]. ECL has high spatial control of electrochemical methods as the reactions take place at the surface of an electrode. The most common ECL luminophore is probably the tris(2,2’bipyridyl)ruthenium(II) (Ru(bpy)32+), which is a red crystalline polypyridyl compound. Many other transition metal polypyridyl complexes generate ECL, the high stability of Ru(bpy)32+ in aqueous solutions and in the presence of oxygen, its high quantum yield and capability to reach excited state in the reaction with a number of important molecules make it a highly exploited ECL luminophore in assays and sensors [5,6]
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