Abstract
The conditions for headspace solid phase microextraction (HS-SPME) analysis of volatile compounds in simulated beef flavour were determined by fractional factorial experimental design based on Taguchi's orthogonal array. Adsorption temperature (30–60 °C), adsorption time (20–60 min), and the type of SPME phase (65 μm PDMS/DVB, 65 μm CW/DVB, 75 μm CAR/PDMS and 50/30 μm DVB/CAR/PDMS) were significant factors affecting total peak area and the number of peaks in the gas chromatogram, while added salt concentration (0–6%) as well as the 2-factor interactions of adsorption temperature with time, salt concentration or SPME phase, did not significantly affect the gas chromatogram. A sensitive and reproducible HS-SPME method was achieved using the following conditions: adsorption at 60 °C for 60 min on 50/30 μm DVB/CAR/PDMS, followed by desorption of extracted volatiles at 250 °C for 3 min and gas chromatographic separation on a DB-5 analytical column.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
