Abstract
ABSTRACTA sensitive indirect competitive enzyme-linked immunosorbent assay(ic-ELISA) method and a gold nanoparticle immunochromatographic strip were developed to detect amantadine (AM) in foods. A novel AM hapten was prepared, and a sensitive monoclonal antibody against AM was developed. The optimum ic-ELISA conditions included a pH of 7.4 and an ionic strength of 1.6% with no organic solvents. The half-inhibition concentration (IC50) value of ic-ELISA was 1.92 ng/ml, with a limit of detection of 0.62 ng/ml. The immunochromatographic test strip method had a visual cut-off value at 5 μg/kg. Chicken samples were spiked with three concentrations of AM (1, 2, and 5 µg/kg) and analysed by ic-ELISA. Good recoveries were obtained (92.3% at 1 µg/kg AM, 116.1% at 2 µg/kg AM, and 91.8% at 5 µg/kg AM).
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