Development of retrotransposons insertion polymorphic markers and application in the genetic variation evaluation of Chinese Bama miniature pigs

Abstract

Retrotransposons are genetic elements that can amplify themselves in a genome and are abundant in many eukaryotic organisms. In this study, we established some new short interspersed nuclear elements (SINE) and endogenous retroviruses (ERV) retrotransposons insertion polymorphism (RTIP) markers based on BLAT alignment tool strategy, and followed by PCR evaluation. We investigated the genetic variations among four subpopulations of Chinese Bama miniature pigs (BM), including BM in national conservation farm (BM-cov), BM inbreeding population (BM-inb) and BM closed Herd (BM-clo) in Guangxi University, and BM in the Experimental pig farm of Yangzhou University (BM-yzu). Genetic distance, polymorphism information content (PIC) and heterozygosity (He) of these markers in four of BM subpopulations were measured. Twelve SINE and twenty-eight ERV polymorphic molecular markers were identified in the four subpopulations. The BM-cov pigs represented the highest He and PIC, which indicated that BM-cov pigs maintain relatively highly genetic diversity. BM-inb pigs represented the lowest He and PIC indicating less variation and a high degree of inbreeding. Microsatellites polymorphism in four BM populations also well supported the results of these RTIP markers. In summary, retrotransposons insertion polymorphic markers could be a useful tool for population genetic variation analysis. Current SINE and ERV variation data may also provide a reference guide for the conservation and utilization of the BM miniature pig resource.