Abstract
The kinetics of the maturation of B cells were studied in adult thymectomized, lethally irradiated, and bone marrow-reconstituted mice. The spleen cells of the recipients were taken at various intervals after transfer and cultured in vitro with trinitrophenylated sheep erythrocytes (TNP-SRBC). The cultures were supplemented with either allogeneic culture supernatant or educated T-cell helper activity. Appearance of functional B cells in the bone marrow inoculum was assessed by the number of hemolytic plaque-forming cells (PFC) on the fourth day of culture. In a parallel series the frequency of surface Ig-bearing cells was determined by using fluorescent rabbit anti-mouse Ig serum. When helped by allogeneic culture supernatants, differentiating bone marrow cells showed a slower rate of maturation into functional B cells than when helped by specifically educated T cells. But in both cases the recovery of responsiveness reached the same level (number of PFC/10 6 cells) as that of normal spleen cells 55 to 60 days after transfer. During the initial periods of recovery, bispecific PFC (reacting both to TNP and to native SRBC determinants) were detected regularly in numbers far exceeding their frequency in normal spleen cell cultures; in some experiments, the number of bispecific PFC amounted to as much as 30% of the total PFC, whereas, when the bone marrow cells completely recovered (sixtieth day), the frequency of bispecific PFC was similar to that found in normal spleen cell cultures. The number of surface Ig-bearing cells also reached a normal level after the fiftieth day following transfer. In general, the degree of functional maturation was better correlated with the cells bearing surface Ig in the shape of rings or caps, whereas the predominance of spot-bearing cells indicated immaturity of the population.
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