Abstract
Mycoplasma pneumoniae developed high degrees of resistance to streptomycin and dependence on streptomycin after serial subculture in broth media containing streptomycin. The streptomycin-dependent organisms failed to grow, as indicated by a color change of the medium, in the absence of streptomycin but grew in limited concentrations of streptomycin in broth media. The minimum growth-inhibitory concentration of streptomycin for the parent sensitive strain was very close to the minimum concentration of streptomycin which permitted the growth of the streptomycin-dependent organism. The broth cultures showing streptomycin dependence were shown to consist of streptomycin-dependent organisms or a mixture of streptomycin-dependent and -resistant organisms, as tested on agar media containing varying concentrations of streptomycin.
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