Abstract
Estimation of therapeutic levels of Imatinib and its correlation with clinical response has become important to monitor response of this drug in chronic myeloid leukemia (CML) patients. Various attempts have been made to develop quantitative protocols for imatinib in human serum using different analytical tools. A simple, sensitive and specific quantitative method was developed in the present study for monitoring of imatinib levels in human serum by applying high performance liquid chromatography (HPLC) and QQQ tandem mass spectrometry. An efficient simple sample extraction method was developed using solid phase extraction (SPE) method for extraction of imatinib in human serum with a recovery of around 80-85%. The developed method was successfully tested on 30 human serum samples who have been receiving imatinib for CML.
 Keywords: Imatinib, LCMS, MRM, methanol, serum
Highlights
Introductionchronic myeloid leukemia (CML) is a clonal myeloproliferative disorder of the primitive hematopoietic stem cell
chronic myeloid leukemia (CML) accounts for approximately 15% of adult leukemias
A simple, sensitive and specific quantitative method was developed in the present study for monitoring of imatinib levels in human serum by applying liquid chromatography (LC) and QQQ tandem mass spectrometry
Summary
CML is a clonal myeloproliferative disorder of the primitive hematopoietic stem cell. It is characterized by presence of balanced genetic translocation of chromosomes 22 and 9, termed as Philadelphia chromosome. Monitoring of trough plasma concentration and clinical response in CML is mainstay of imatinib therapy. There are various studies which shows that there is correlation between trough plasma concentration and clinical response in CML. Imatinib is an antineoplastic agent and the molecular weight of the compound is 493.6 g/mol. A trough level close to 1000ng/ml, appears to be correlated with better cytogenetic and molecular responses. In the present study simple, specific and precise method was developed with simple sample preparation steps for extraction of imatinib from serum
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