Abstract
Twelve polymorphic microsatellite markers from Ancherythoculter nigrocauda were developed using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. These loci were characterized by genotyping 48 individuals. The observed number of alleles per locus ranged from 4 to 14 with an average of 9.4. The expected and observed heterozygosities ranged from 0.185 to 0.907 and from 0.083 to 0.952, respectively. Among these polymorphic microsatellite loci, four of the loci (HWB01, HWB04, HWB12 and HWB16) significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction (P < 0.05). All of the microsatellite loci were in linkage equilibrium. These microsatellite markers would be useful for investigating the genetic diversity and population structure of A. nigrocauda.
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