Abstract
We have developed a polydiacetylene (PDA)-based sensing platform to detect testosterone (T) as a potential biomarker of preterm birth. The insolubility of the steroid hormone in water, where PDA assemblies are dispersed, poses a major issue, since they can hardly interact with each other. To overcome this challenge, acetonitrile was used as a suitable solvent. In addition, to minimize false signals of PDA assemblies caused by the solvent, a mixture of acetonitrile and distilled water was selected. To prove a concept of PDA-based sensing platform for targeting T hormone, we conjugated anti-T antibodies to surface of PDA assemblies to induce selective binding between T and anti-T antibodies. The fluorescence sensory signaling of the PDA-anti-T antibody conjugate was selectively generated for T, over 3.4 times higher sensitivity of the signaling compared to that from other sex steroid hormones studied (β-estradiol and progesterone).
Highlights
Accepted: 8 July 2021The low birth rate of developed countries is a growing concern, mainly because it will cause national productivity to plummet in the near future
We developed three types of pentacosadiynoic acid monomers (PDA)-based sensing platforms to compare sensory signals in the presence of T: (1) PDA liposomes consisting of Pentacosadiynoic acid (PCDA) monomers (PDA), (2) albumin conjugated to PDA (PDA-Albumin), and (3) anti-T
We developed three types of PDA-based sensing platforms to compare sensory signals in the presence of T: 1) PDA liposomes consisting of PCDA monomers (PDA), 2) albumin conjugated to PDA (PDA-Albumin), and 3) antiT antibody conjugated to PDA (PDA-T Ab) (Figure 1b)
Summary
The low birth rate of developed countries is a growing concern, mainly because it will cause national productivity to plummet in the near future. Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. The liposomes consisting of amphiphilic diacetylene or/and lipid molecule are readily formulated in aqueous and the surface of the liposomes could be immobilized with various biomolecules such as peptide, enzyme and antibody [15,16,17,18,19,20] These characteristics of simple formulation and easy-to-surface modulation enable for the PDA liposomes to be used as accessible and economical sensing platform. Namely T, progesterone, and β-estradiol, were screened and incubated to generate sensory signals with PDA assembly of albumin or anti-T antibody conjugates via colorimetric and fluorogenic transitions
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