Abstract
Our study aimed to prepare nanoemulsions coated with alginate/Aloe vera gel (A/AV) for oral insulin delivery. Uncoated nanoemulsions were prepared by homogenization of water in oil in water (w/o/w) multiple emulsions that were composed of castor oil (emulsifier). Coating of the nanoemulsions was achieved based on ionic gelation method, with sequential addition of calcium chloride and Aloe vera gel (AV) to the bulk nanoemulsion dispersion that contained alginate. The particle size of the coated nanoemulsions was about 400 nm and the insulin entrapment ratio was 47.3%. In vitro leakage study indicated well-preserved integrity of the nanoemulsions in simulated gastric juices. In conclusion, the nanoemulsion coated with alginate/Aloe vera gel (A/AV) was a potential delivery system for oral delivery of polypeptides and proteins. Feasibility of this nanoemulsion for oral delivery of insulin (model peptide) is investigated in Caco-2 cell line using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide viability assay. The cell culture results for translocation of insulin across the cell monolayer are very promising (20%–25% increase), and animal studies are actively under progress and will be published separately in near future.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
