Development of Murine Model for Breast Cancer Metastasis to Bone

Abstract

Breast cancer is the second most common diagnosed cancer. 70% of breast cancers metastasize to the bone. This metastasis is more serious than the original tumor. The present study deals with the generation of animal (rat) model for breast cancer metastasis to bone by insertion of human breast cancer cell line MDA-MB 231. The cell line MDA-MB 231 was administered in the immunosupressed rat femur bone and tumor formation was observed for two months. Blood parameters viz; SGOT, SGPT, ALP, TRAP and calcium and antioxidant enzymes (catalase, SOD, GSH, GSSG, GST, MDA and GPX) in liver tissue were studied after one month and two months of insertion. In our study we observed increase in the antioxidant liver enzyme levels i.e. Catalase, SOD, GSH, GSH and MDA, and SGOT, ALP, TRAP and calcium levels also were increased in serum. Increased levels of antioxidant enzymes are indicative of increases Reactive oxygen species (oxidative stress) are involved in initiation, promotion and progression of carcinogenesis. Further, bone histopathology results indicate necrotic bone in experimental rats, which suggests osteolytic bone metastasis. This developed model for breast cancer metastasisto bone is cost effective and gives insight into the metastatic colonization, in which initially formed micrometastasis succeed in colonizing the marrow and generating osteolytic metastasis.