Abstract
Goose parvovirus (GPV) causes high mortality in domestic goslings and Muscovy ducklings which affects geese production worldwide. In this study, loop-mediated isothermal amplification (LAMP) was developed with two pairs of specific primers to detect GPV. The established method proved to be highly sensitive to detect one copy of GPV genome as same as polymerase chain reaction (PCR). No cross-reaction with other tested goose pathogens was shown in specificity test. The results of detecting fifty clinical specimens and three preserved GPV strains by LAMP assay suggested that the method was well consistent with PCR. The developed LAMP is a simple, fast method for the detection of GPV infection. Key words: Goose parvovirus (GPV), loop-mediated isothermal amplification (LAMP), detection, comparison, polymerase chain reaction (PCR).
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