Abstract
Testes material from 16 men was used to study the development of human meiosis in vitro. The present report deals with the results from the analysis of the cells from the organ cultures of five of these men. H3-thymidine was added to the culture medium for 75 to 90 minutes. The isotope was removed and the cells were thoroughly washed in 3 changes of fresh medium without tritium. Subsequently they were incubated at 36°C for 14 days. Feulgen squashes and sections were prepared and labelled cells were photographed. After this procedure the silver grains were removed from the autoradiographs in order to identify the meiotic stages. The most advanced stages found labelled, after the cells had been in culture for 14 days, were the following: prometaphase I, metaphase I, prophase II and telophase II. Under the culture conditions used human spermatocytes are able to develop in vitro from DNA synthesis to the end of the second division of meiosis.
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