Development of in-vitro propagation technique for onion (Allium cepa L.) regeneration

The study was conducted to investigate the effect of different concentration of indole acetic acid (IAA), naphthalene acetic acid (NAA) and gibberellic acid (GA3) on okra pod setting and size, soluble solids content (SSC), vitamin C and mineral contents using flower ovary injection method. The fruit set significantly increased in IAA and GA3 compared to the NAA at 100mg/L concentration. NAA had lower fruit setting than the IAA and GA3 treated plant. The higher pod (fruit) size was obtained in GA3 (18.8 cm²) as compared to the IAA (8.98) and NAA (8.72 cm²) at 100 (mg/L) of concentration. Moreover, the highest SSC was obtained by 100 mg/L of IAA concentration as compared to the GA3 and NAA. The maximum vitamin C was found in IAA and GA3 as compared to 100 mg/L NAA concentration. On the other hand, the higher mineral contents like K, Ca, Mg, Na and Fe was found in 100 mg/L IAA and GA3 concentrations than 100 mg/L NAA concentration. The higher concentrations (100 and 200 mg/L) of IAA and NAA greatly decreased the healthy seed percentage as compared to the lower concentration. However, GA3 increased the healthy seed percentage at 50 and 100 mg/L then decreased at 200 mg/L concentration. Aborted seed percent was increased from 25-100mg/L IAA and NAA and then decreased at 200 mg/L. however it was found increasing trend from 25-200mg/L GA3 concentration. It seemed that GA3 and IAA were better growth regulator as compared to the NAA for okra pod production and development of nutrient content.

Tulip (Tulipa gesneriana L.) is an important and highly valuable flower of the cut flower industry. The most critical step in its cultivation is to break dormancy in order to initiate the growth, especially in tropical and sub-tropical areas of the world. Therefore, the current research was conducted to break bulb dormancy and foster the growth of tulip in Potohar region with the help of different primers. The objective of this study was the selection of best primer at appropriate concentration level to enhance growth, yield and vase life of the flower. Tulip bulbs were treated with different primers: T0 (distilled water), T1 (chitosan @ 5 g/L), T2 (gibberellic acid @ 0.15 g/L), T3 (humic acid 160 g/L), T4 (imidacloprid 19 g/L) and T5 (salicylic acid 0.1 g/L) for 24 hours, respectively. The experiment was laid out using Complete Randomized Design (CRD) with three replications. Statistical results revealed that characteristics including early germination, plant height, number of leaves, stalk length, fresh and dry weight of flower, weight of bulbs, diameter of bulbs and number of daughter bulbs were significantly increased in T2. Whereas, leaf area, diameter of stem and flower was maximum in T0. Plants under T3 showed an increase in chlorophyll content of leaves. While floral characteristics like early formation and opening of flower bud, more number of flowers and vase life were improved in T1. Thus, statistical results showed that priming can effectively help to improve morpho-physiological attributes of tulip.

Interactive effects of α-NAA and UV-B radiation on the endogenous hormone contents and growth of Trichosanthes kirilowii Maxim seedlings

Bigtooth maple (Acer grandidentatum) is a promising ornamental tree that is not widely used in managed landscapes. Tissue culture has not been used successfully to propagate this taxon. We cultured single- and double-node explants from greenhouse-grown, 2-y old seedlings of bigtooth maples, which are indigenous to New Mexico, Texas, and Utah, on Murashige–Skoog (MS), Linsmaier–Skoog (LS), Driver–Kuniyuki Walnut (DKW), and Woody Plant (WPM) tissue culture media. Media affected shoot proliferation (P = 0.0242) but the zone of explant origin (P = 0.7594) did not. After four 30-d subcultures, explants on DKW media and WPM media produced 3.6 and 3.5 shoots per explant, respectively. Sprouting rates were highest on DKW, making DKW the best overall media for shoot proliferation. Double-node microshoots were rooted in vitro on DKW containing indole acetic acid (IAA). Microshoots represented six genotypes from three locations within Texas and New Mexico. Rooting percentage increased up to 15% as IAA concentration increased (P = 0.0040). There was 100% survival of rooted microshoots in vented Phytatrays containing one perlite: one peat moss (v/v). We conclude that DKW can be used to proliferate microshoots, and IAA induces rooting in microshoots of bigtooth maple.

The wounds, its depth and the time of inoculationof Agrobacterium tumefaciens on castor bean )Ricinus communis( plants affecting on the development of apricot crown gall disease whereas, the increase of wound depth and adding the pathogen after directly wounding plants increased the disease severity and gall formation. Where, the highest infection and gall formation resulted from inoculation of A. tumefaciens after directly wounding plants (zero time) at 3mm depth followed by 5mm depth, while the lowest gall formation and disease severity showed when adding A. tumefaciens suspension after 48 hrs. from wounding at one mm depth. On apricot plants (Prunus armeniaca), The plant hormones indole acetic acid (IAA) and cytokinin (CK) were decreased in the first stage of gall formation, while they were increased in the other stages of gall formation. Gibberellic acid (GA) was decreased in the first stage and then increased but it decreased again in the last stage. On the other hand, abscisic acid (ABA) inactivation plant hormone showed an opposite result whereas, ABA was increased in the first stage of gall formation and then it was decreased in the other stages of disease. Also, A. tumefaciens isolates obtained from different hosts having different gall index (different in virulence) produced different rates of plant hormones IAA, GA, ABA and CK. But the rate of CK was found to be correlated with virulence. Also, crown gall disease development dependent on the production amount of the three plant hormones (IAA, GA3 and CK) and the ratio between them. Fertilizers with (NPK) and compost resulted in decrease the severity of crown gall disease on apricot plants whereas; they reduced the number and weight of galls and root gall index in the treated plants compared with control. The best one treatment was (NPK) then (NP) and (NPK plus compost). Fertilizers with (NPK plus compost) due to increase the foliage of plants and in all fertilizer treatments increased the roots of plants compared with control except that treated with (NP). Also, no variation considered in the height of plants in all treatments, but the highest height recorded from (NPK plus compost) treatment. On the other hand, the uptake of (N) was increased in all fertilizers when used (N) alone or in combination with (P or K), also, (P) up take increased when used (P) alone or with (N or K). But (K) up take was increased only when used it with (N) or (P) and in combination with (NPK)

Studies were carried out to investigate the regeneration and rapid multiplication of Withania somnifera (L.) in vitro. Direct and indirect regeneration protocols for multiple shoots development from epicotyl explants of 50 to 60 days old seedlings were established. The shoots were initiated directly from epicotyl explant on 6-benzyl amino purine (BAP: 2.0 mg/L) along with indole-3-acetic acid (IAA: 0.2 mg/L), and the maximum of 15.5 ± 0.90 shoots/explant were achieved by subsequent subcultures at 4 weeks interval in the same medium. Calli (98.3%) were produced from epicotyl explant on 2,4-dichlorophenoxy acetic acid (2,4-D: 2.0 mg/L) along with kinetin (Kn: 0.6 mg/L), and shoots were initiated from calli on BAP (1.0 mg/L) along with adenine sulphate (AdS: 20.0 mg/L). Proliferation of shoots was achieved by subsequent subcultures at 4 weeks interval in the same medium. The maximum value of 25.3 ± 1.81 shoots/explant was achieved in the second subculture of indirect regeneration. Murashige and Skoog (MS) medium along with gibberellic acid (GA3) at 1.0 mg/L produced maximum 73.3 and 95.5% of shoot elongation in direct and indirect regenerated shoots, respectively. On the other hand, MS medium with indole-3-butyric acid (IBA) at 0.8 mg/L induced maximum 86.7% and 90.0% of rooting from elongated shoots of direct and indirect regeneration, respectively. The rooted plants were transferred to small cups filled with sterilized mixture containing soil, sand and vermiculite (1:2:1, v/v/v) for hardening. About 90% of plants survived in the hardening process, and then the plants were established successfully in the experimental field. This protocol yielded a higher number of shoots within a short period. Consequently, the protocol developed in this study offers a simple and improved in vitro method to regenerate W. somnifera. Key words: Withania somnifera, epicotyls, tissue culture, shoot elongation, root induction.

Filipendula ulmaria (L.) Maxim (meadowsweet) is a medicinal plant that is claimed to have several biological activities, including anti-tumor, anti-carcinogenic, anti-oxidant, anti-coagulant, anti-ulcerogenic, anti-microbial, anti-arthritic, and immunomodulatory properties. This report describes, for the first time, an efficient plant regeneration system for F. ulmaria via adventitious shoot development from leaf, petiole, and root explants cultured on Murashige and Skoog’s minimal organics medium containing different concentrations of thidiazuron (TDZ), benzyladenine, and kinetin either alone or in combination with different auxins. Relatively extensive/prolific shoot regeneration was observed in all three explant types with TDZ in combination with indole-3-acetic acid (IAA). Gibberellic acid (GA3), TDZ, and IAA combinations were also tested. The best shoot proliferation was observed among root explants cultured on media supplemented with 0.45 μM TDZ + 2.85 μM IAA + 1.44 μM GA3. Regenerated shoots were transferred to rooting media containing different concentrations of either IAA, indole-3-butyric acid (IBA), naphthalene acetic acid, or 2,4-dichlorophenoxyacetic acid. Most shoots developed roots on medium with 2.46 μM IBA. Rooted explants were transferred to vermiculite in Magenta containers for a 2-wk acclimatization period and then finally to plastic pots containing potting soil. The plantlets in soil were kept in growth chambers for 2 wk before transferring to greenhouse conditions.

Sorbus aucuparia L. is an important forest tree used in the reforestation of high-altitude mountainous areas, which carries significant pharmaceutical, industrial, ornamental, food, and ecological properties. However, the seed propagation and micropropagation of mature trees of S. aucuparia L., presents various difficulties which are integral aspects for the re-propagation and breeding of novel cultivars. In this study, we isolated the mature embryo-containing seeds of selected varieties of S. aucuparia L. from their seed coat and used them directly as explants in vitro to investigate and determine the optimal dose of cytokinin in breaking seed dormancy and micropropagation. 3 mg/L of benzyl adenine (BA) in addition to different concentrations and combinations of kinetin (0.5, 1, and 2 mg/L), indole-3-butyric acid (IBA) (0, 1, 0.5, and 1 mg/L), and 1-naphthalene acetic acid (NAA) (0, 1, 0.5, and 1 mg/L) were applied within a Murashige and Skoog (1962) (MS) medium at germination inhibition, shoot elongation, and shoot proliferation. In the MS mediums containing BA and kinetin, germination was achieved at the end of 1 week, and shoot proliferation was achieved at the end of 3 weeks. The most successful germination (96%), tallest shoot length (mean 5.1 cm), most shoot proliferation (mean 7.2 pieces), and number of nodes (mean 9.7 pieces) were identified in the MS containing 3 mg/L BA and 1 mg/L kinetin. Direct root formation with shoot elongation occurred in 25% of explants which germinated in the MS medium. For shoots propagated without roots in this medium, 62% of these achieved rooting at the highest dose of 1 mg/L indole-3-acetic acid (IAA) using a two-stage rooting method. Rooted shoots were successfully transferred to an ex vitro medium. These results provide a basis for breaking seed dormancy of selected Sorbus L. genotypes quicker, leading to more effective clonal production. Keywords: Sorbus aucuparia L., rowanberry, tissue culture, seed dormancy, micropropagation, in vitro

Green gram (Vigna radiata) is considered the chief legume in Pakistan. Thus, current study was conducted to examine the ameliorating effect of phytohormones pre-treatments under salt stress on proteome profile of green gram by sodium-dodecyl-sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The soluble green gram seedlings proteins were resolved on 4% stacking and 12% resolving gels. The SDS-PAGE resolved 24 polypeptide bands ranging from 200 to 17kDa. Among these, 12 out of 24 bands of proteins were essentials house-keeping or growth proteins of green grams. While, 120, 114.6, 51.8, 29.1, and 22.8 kDa bands were over-expressed under 50 to 350mM salt with phytohormones treatments. The others 104.5 kDa, 99.8 kDa, 95.3 kDa, 91.0 kDa, 55 kDa, 46 kDa, and 17kDa bands were related to the GAᴣ, IAA, and SA induced tolerance. Overall 120 kDa, 114.6 kDa, 104.5 kDa, 99.8, 95.3 kDa, 51.8 kDa, 29.1 kDa and 22.8kDa bands were first time identified in the current study. The information retrieved from NCBI protein database, the resolved peptides were principally belonging to 7S and 8S vicilin, 2S, 8S, 11S, and 16.5S globulins. It is determined that seed priming with SA enhanced tolerance in green gram by rapidly synthesizing stress alleviating peptides.Key word: Cluster analysis, dendrogram, mungbean, salt stress, SDS-PAGEINTRODUCTIONVarious world-wide health concerning organization recommended the use of high graded plant protein such as legumes to prevent the risk of metabolic disorder (Hou et al., 2019). Legumes are most important protein crop on the earth. Among the legumes, the green gram is the major pulses. Its seeds are rich in superior quality storage protein, which account 85% of the total protein while, another 15% have not been broadly studied (Yi-Shen et al., 2018). The soluble storage protein comprises of 60% globulins, 25% albumin and 15% prolamins. Globulins are further divided into 3.4% basic-type (7S), 7.6% legumin-type (11S), and 89% vicilin-type (8S) (Mendoza et al., 2001; Itoh et al., 2006). Other than proteins, the green gram seeds also contain starch, fiber, phenolic compound, saponins, vitamins, calcium zinc, potassium, folate, magnesium, manganese and very low in fat that made it meager man’s meat (Hou et al., 2019). It is also a good source of green manure and fodder (El-Kafafi et al., 2015). Its root has ability to fix 30 to 50 Kg/ha atmospheric nitrogen in the soil which is essential for maintaining soil fertility (Chadha, 2010). The green gram is the valuable and the major Rabi pulse crop of Pakistan. Its cultivation area in 2016-2017 was about 179,000 hectares with seed yield of 130,000 tones. In comparison during 2017-2018, it was cultivated on 161,800 hectares land with 118,800 tones seed yield (GOP, 2018). One of the reasons of this 9% decrease in both land and productivity is the shortage of irrigated land due to soil salinity. The salinity induce oxidative bust in the mungbean cells, caused by responsive oxygen species (ROS) such as hydrogen peroxide, singlet oxygen, hydroxyl radical and superoxide radical. The ROS create hindrance in various metabolic processes of plant via interacting with macromolecules like proteins (Alharby et al., 2016). However, phytohormones like gibberellic acid (GAᴣ), indole acetic acid (IAA), and salicylic acid (SA) take part in the biosynthesis of salt tolerance proteins under salinity. These salt tolerance proteins acclimate plants under salinity stress. Application of biotechnology plays a significant role in agriculture (Khan et al., 2017). Therefore, production of particular proteins under salt stress is a specific response of cell which can be analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE is the simple, valid, and cost-effective biochemical marker (Mushtaq et al., 2018). This marker has been widely used to determine the extent of evolutionary variations in crops (El-Kafafi et al., 2015).OBJECTIVES The present study was directed first time with the aim to investigate the toxic effect of sodium chloride (0-350 mM) and stress acclimation by pre-treatment of GAᴣ, IAA, and SA on the proteome profile of NM-92 cultivar of a Pakistani green gram.MATERIALS AND METHODSThe present study was replicated thrice in the plant laboratory of Department of Genetics, Faculty of Science, and University of Karachi. The seeds of mung bean cultivar NM-92 were acquired from National Agricultural Research Centre (NARC), Islamabad. These freshly collected 15 seedsˉ1 treatment / replication were divided into two sets. The first was named as sodium chloride (SC) stress treatments were imbibed in sterile distilled water (DW) whereas, second set soaked in gibberellic acid (GAᴣ) (BDH Chemicals, England), indole acetic acid (IAA) (Fluka, Switzerland), and salicylic acid (SA) (J.T. Baker, Holland) in the separate beaker for 24 hours under dark condition. After 24 hours, given ample time to both the sets at room temperature. After recovery, all 20 treatments were sown in the 150 X 30 mm sized petri-dishes containing 0, 50, 150, 250, and 350 millimolar (mM) sodium chloride solution (Fisher Scientific, UK) for 72 hours.Protein extraction: Protein extraction was done by taking 0.3g of seedlings in an ice chilled mortar and crushed by adding 600µL 0.2 M Tris-HCl buffer having pH 7.5 contained 5% SDS (w/v) and 5% 2-mercaptoethanol (v/v). The homogenate was incubated at 0oC for 30 min., boiled in the water bath for 3 min. at 100oC. Samples were centrifuged in Heraeus Biofuge D-37520, Germany for 30 min. at 8000 rpm. The protein supernatant was saved at below 0°C for quantitative and qualitative determination with minor modifications. The total soluble protein content of the samples was estimated via “Bovine Serum Albumin (BSA) standard curve” and explicit in µg protein milligramˉ1 fresh weight of mung seedlings.Bovine serum albumin standard curve (2000 μg/mL): Total protein standard curve was made by dissolving 0.05g of Bovine Serum Albumin (BSA) in 25mL of distilled water. Ten serial dilutions were made from 0.1 mL to 1mL by BSA solution then performed Lowry. A standard curve of total proteins was plotted by taking BSA absorbance at Y-axis and 2000 μg BSA / mL at X-axisSample preparation for SDS-PAGE: For qualitative assessment of total proteins; the 35μL of saved protein supernatant was combined with 15μL of sample diluting buffer (SDB). The SDB was made up of 0.0625 M Tris-HCl pH 6.8 with 2% of SDS, 10% of glycerol, 0.003% of bromophenol blue dye and 5% of 2-mercaptoethanol. Boil the 50μL protein SDB supernatant at 100oC in water bath for 3 min., centrifuged at 6000 rpm for 4 min. The supernatant was loaded on SDS-PAGE gel with the given formulae. The SDS- PAGE: Total proteins were fractionated via SDS-PAGE with 4% stacking and 12% resolving gel. The resolving gel of 12% was made by taking 6mL solution A, 1.8 mL 3 M Tris 1 M HCl buffer pH 8.8, 144μL 10% SDS, 5.74 mL sterile distilled water, 720μL 1.5% ammonium persulphate (APS) in deionized water and 10μL TEMED. While, stacking was composed of 1.25mL of solution A, 2.5mL of 0.5M Tris 1M HCl buffer pH 6.8, 100μL 10% SDS, 1.8 mL of distilled water, 500μL 1.5% APS and 12μL TEMED. Solution A was prepared by conjoining 30% acrylamide and 0.8% N, N’-methylene-bisacrylamide in deionized water. To avoid polymerization in the beaker; the prepared solution was quickly poured into the 3 mm thick gel plates after adding TEMED. The stacking was lined over resolving gel, then combs were inserted between the gel plates of SCIE-PLAS TV-100 separation system, UK, and allowed to polymerize for ½ an hour. After polymerization gel was placed in the tank which were filled with Tris-Glycine buffer (electrode buffer) pH 8.4 then combs were removed. The electrode buffer contained 0.3% Tris, 1.41% Glycine and 0.1% SDS in 2000mL d/w. The gel was pre-run for 15 min. at 60 volts and 120 mA currents. The prepared SDS-PAGE samples were loaded in wells with BlueStepTM Broad Range Protein Marker, AMRESCO, USA as standard and run at 60 volts & 120 mA for about 45 min. When samples entered in resolving gel, and then gave 100 volts and 200 mA currents for around 2.5 hours. Furthermore, electrophoresis was carried out at a constant watt.The Gel was washed with 30% ethanol on Uni Thermo Shaker NTS-1300 EYELA, Japan at the constant shaking for 30 min. Then gels were placed in 10% glacial acetic acid in 50% methanol solution (Fixative) for 24 hours. SDS Gel was stained until protein bands were visible thereat placed as 5% of Methanol in 7.5% acetic acid glacial solution to destain the bands background. SDS-PAGE stain composed of 0.125% coomassie brilliant blue R-250 dissolved in 40% of Methanol and 7% acetic acid glacial solution. The stain was stirred on Magnetic stirrer & hot plate M6/1, Germany for 6-10 hours before used. Photographs were taken by Sanyo digital camera VPC-T1284BL and bands were scored through numbering pattern. Gels preserved in 10% acetic acid solution at 4°C.Interpretation of bands and data analysis: The total soluble protein bands relative mobility calculated by below formulae and Dendrogram was constructed via SPSS v. 20Where,F=(Migrated distance of protein band)/(Migrated distance of dye front)Slop=(Log MW of protein marker lower limit band–log〖MW of protein marker upper limit band )/(RF protein marker lower limit band –RF of protein marker upper limit band)RESULTS:The total soluble proteins extracted from green gram were perceived by SDS-PAGE Blue StepTm broad range biochemical markers. The protein-based marker was used to evaluate the toxic effect of sodium chloride along with pre-treatments of GAᴣ, IAA, and SA on proteome assay. In the current work, seedlings total soluble proteome resolved 24 polypeptide bands ranging from 200 to 17.1 kDa were recognized by using SDS-PAGE. The figure 1 showed Dendrogram assay, which classified the 20 treatments of SC, GAᴣ, IAA and SA into two major cl

Root Formation on Petioles of Detached Primary Leaves of Dwarf Bean (Phaseolus vulgaris) Pretreated with Gibberellic Acid, Triiodobenzoic Acid, and Cytokinins

A protocol for micropropagation of Arbutus andrachne from seeds was developed. Results indicated that none of the seeds cultured on Murashige and Skoog (MS) medium, with or without plant growth regulators (PGRs), germinated. Seeds soaked in 250 mg l−1 gibberellic acid (GA3) at 4°C for 3 days, then cultured on water-agar medium containing 2.0 mg l−1 GA3 exhibited 80–100% germination and developed into usable seedlings. Shoot proliferation was tested on MS or B5 medium containing different concentrations of cytokinin. No shoot proliferation was observed on PGR-free medium. Proliferation was more successful on MS than on B5 medium. On both media, the most successful proliferation was obtained using zeatin as a cytokinin type. Rooting was tested on MS medium containing different concentrations of auxin. Rooting failed on PGR-free medium and on medium containing indole-3-acetic acid (IAA), 0.25 or 0.5 mg l−1 indole-3-butyric acid (IBA), or 0.25, 0.5 or 2.0 mg l−1 α-naphthaleneacetic acid (NAA). Rooting (40%) was most successful with 1.0 mg l−1 NAA. Rooted plantlets exhibited 80% survival in all mixtures of peatmoss and perlite, and acclimatized plants were successfully grown in the greenhouse. Quantitative analysis of arbutin performed on in vivo and in vitro leaves using high-performance liquid chromatography (HPLC) revealed that in vivo leaves contained higher arbutin content (0.3–0.81% w/w) than in vitro leaves (0.09% w/w). The highest yield of arbutin in vivo was detected in leaves collected in August, and the lowest yield in leaves collected in December.

Lead (Pb) is a potential pollutant that readily accumulates in soils and sediments widely occurred as a result of human, agricultural and industrial activities. IAA and NAA belongs to plant hormone auxin that promote plants growth. The effects of lead (Pb) phytotoxicity include stunted growth, chlorosis, blackening of the root systems, alteration in water and nutritional status of plants, inhibition of enzyme activities and so on. This study therefore aim at examining possible ameliorative effects of Indole acetic acid( IAA) and Naphthalene acetic acid (NAA) on some biochemical parameters (photosynthetic pigments, mineral elements and antioxidant enzymes) of soybean exposed to lead( Pb) toxicity. One thousand (1000)mg/L of PbNO3 was applied to 5kg of soil in a polythene bag It was allow to acclimatize for 2 weeks after which the seed were sown in the soil. Fourty (40)mg/L, 80 mg/L and 120mg/L each of IAA and NAA were prepared and applied by foliar method after 5 weeks of planting. The plants were harvested after 7 weeks and was partitioned into root, stem and leaf for analysis. The results showed that the level of chlorophyll a, b and carotenoid, mineral elements and antioxidant enzymes significantly increased (P˂0.05) in the root, stem and leaf of soybean treated with different concentration of Indole acetic acid (IAA) and Naphthalene acetic acid (NAA) as compared with the control (C+ve). All bioregulators (IAA and NAA) concentrations significantly decreased (P˂0.05) in the level of Pb2+ (except 40mg/L IAA in the root and leaf while all concentration have significant effects in the stem) and also all concentration of (IAA and NAA) significantly increased (P˂0.05) in the level of biochemical parameters to different extent as compared to control(C+ve). These results shows that Indole acetic acid (IAA) and Naphthalene acetic acid ( NAA) could ameliorate the Pb toxicity and therefore will be of relevance to agricultural producers .

Lead (Pb) is a potential pollutant that readily accumulates in soils. IAA and NAA belongs to plant hormone auxin that promote plants growth. This work aim at examining possible ameliorative effects of Indole acetic acid (IAA) and Naphthalene acetic acid (NAA) on some biochemical parameters of soybean exposed to Pb toxicity. Seeds of soybean (TGX 1835-10E) genotype were sown in 5kg bags of soil containing 1000mg/L of Lead nitrate (PbNO3). 40mg/L, 80 mg/L and 120mg/L each of IAA and NAA were prepared and applied by foliar method after 5 weeks of planting. Photosynthetic pigments, mineral elements and antioxidant enzymes were determined on the root, stem and leaf after 7 weeks of planting. Data were analysed using anova at 5% level of significance. The results showed that the level of chlorophyll a, b and carotenoid, mineral elements and antioxidant enzymes significantly increased in the root, stem and leaf of soybean treated with different concentration of Indole acetic acid (IAA) and Naphthalene acetic acid (NAA) as compared with the control (C+ve). All bioregulators (IAA and NAA) concentrations significantly decreased in the level of Pb2+ (except 40mg/L IAA in the root and leaf while all concentration have significant effects in the stem) and also all concentration of (IAA and NAA) significantly increased in the level of biochemical parameters to different extent as compared to control(C+ve). These results show that Indole acetic acid (IAA) and Naphthalene acetic acid (NAA) could ameliorate the Pb toxicity and therefore will be of relevance to agricultural producers.

Cytoplasmic male sterility (CMS) provides an irreplaceable strategy for commercial exploitation of heterosis and producing high-yielding hybrid rice. The exogenous application of plant growth regulators could improve outcrossing rates of the CMS lines by affecting floral traits and accordingly increase hybrid rice seed production. The present study aimed at exploring the impact of growth regulators such as gibberellic acid (GA3), indole-3-acetic acid (IAA), and naphthalene acetic acid (NAA) on promoting floral traits and outcrossing rates in diverse rice CMS lines and improving hybrid rice seed production. The impact of foliar applications of growth regulators comprising GA3 at 300 g/ha or GA3 at 150 g/ha + IAA at 50 g/ha + NAA at 200 g/ha versus untreated control was investigated on floral, growth, and yield traits of five diverse CMS lines. The exogenously sprayed growth regulators, in particular, the combination of GA3, IAA, and NAA (T3) boosted all studied floral, growth, and yield traits in all tested CMS lines. Moreover, the evaluated CMS lines exhibited significant differences in all measured floral traits. L2, L3, and L1 displayed the uppermost spikelet opening angle, duration of spikelet opening, total stigma length, style length, stigma brush, and stigma width. In addition, these CMS lines exhibited the highest plant growth and yield traits, particularly under T3. Consequently, exogenous application of GA3, IAA, and NAA could be exploited to improve the floral, growth, and yield traits of promising CMS lines such as L2, L3, and L1, hence increasing outcrossing rates and hybrid rice seed production.

The formation of new xylem in the spring is preceded by bud development. In decapitated pine stem the formation of xylem is arrested until the outgrowth of interfascicular buds takes place. When indole‐3yl‐acetic acid (IAA) is applied to the cut surfaces of decapitated stems it induces the formation of a xylem ring on the whole length of 5‐ycar old trees. Naphthaleneacetic acid (NAA) causes the formation of xylem; however, the width of the growth ring is several times broader at the point of application than at the base of the leader. Cis‐ and trans‐cinnamic acids, coumarin, L‐tryptophan, kinetin (Kin), benzylaminopurine (BAP) and gibberellic acid (GA) alone do not induce cambial divisions; however, GA and the cytokinins given jointly with IAA or NAA accelerated the basipetal stimulus which has been induced by the auxins, resulting in normal xylem formation. 2,3,5‐Triiodobonzoic acid (TIBA) given jointly with IAA‐induced formation of compression wood in the apical part of the stem and narrow diameter tracheids at the base. When carboxyl labelled IAA or NAA are applied to pine segments it is found that the basipetal movement of IAA is much quicker than that of NAA. GA and the cytokinins increase the rate of transport of both auxins, whereas TIBA arrests the bulk of auxin in the apical part of the stem.