Abstract
BackgroundLilac (Syringa oblata) is an important woody plant with high ornamental value. However, very limited genetic marker resources are currently available, and little is known about the genetic architecture of important ornamental traits for S. oblata, which is hindering its genetic studies. Therefore, it is of great significance to develop effective molecular markers and understand the genetic architecture of complex floral traits for the genetic research of S. oblata.ResultsIn this study, a total of 10,988 SSRs were obtained from 9864 unigene sequences with an average of one SSR per 8.13 kb, of which di-nucleotide repeats were the dominant type (32.86%, 3611). A set of 2042 primer pairs were validated, out of which 932 (45.7%) exhibited successful amplifications, and 248 (12.1%) were polymorphic in eight S. oblata individuals. In addition, 30 polymorphic EST-SSR markers were further used to assess the genetic diversity and the population structure of 192 cultivated S. oblata individuals. Two hundred thirty-four alleles were detected, and the PIC values ranged from 0.23 to 0.88 with an average of 0.51, indicating a high level of genetic diversity within this cultivated population. The analysis of population structure showed two major subgroups in the association population. Finally, 20 significant associations were identified involving 17 markers with nine floral traits using the mixed linear model. Moreover, marker SO104, SO695 and SO790 had significant relationship with more than one trait.ConclusionThe results showed newly developed markers were valuable resource and provided powerful tools for genetic breeding of lilac. Beyond that, our study could serve an efficient foundation for further facilitate genetic improvement of floral traits for lilac.
Highlights
Lilac (Syringa oblata) is an important woody plant with high ornamental value
Frequency and distribution of Expressed sequence tag (EST)-Simple sequence repeats (SSR) in S. oblata In this study, 104,691 unigenes with a total length of 89.3 Mb were scanned by the Simple Sequence Repeat Identification Tool (SSRIT) [34], and 10,988 potential expressed sequence tag-SSR (EST-SSR) were discovered from 9864 (9.4%) unigenes, with an average of one SSR per 8.13 kb
977 unigenes contained more than one EST-SSR loci
Summary
Lilac (Syringa oblata) is an important woody plant with high ornamental value. The traditional cross-breeding plays an important role in improving the ornamental traits of lilac, it can not adapt to the rapid development of modern lilac industry because of the long breeding cycle and the large amount of resources needed to cultivate new varieties [5]. Little is known about the genetic structure of important ornamental traits of S.oblata, such as florescence, petal color and inflorescence etc. It implies that there is an urgent need to understand the genetic structure of the important traits and shorten the lilac breeding cycle by modern tools to enhance breeding process
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
