Abstract
Background Our aim is to develop replication-competent viral vectors for mucosal delivery, which express HIV-Env immunogens that closely mimic the trimeric glycoprotein spike found on HIV particles. We have developed attenuated recombinant CDV (rCDV) expressing SIVmac239-Env and shown that this vector can be used safely to elicit Env-specific immune responses in ferrets and non-human primates through intranasal administration.
Highlights
Our aim is to develop replication-competent viral vectors for mucosal delivery, which express HIV-Env immunogens that closely mimic the trimeric glycoprotein spike found on HIV particles
We have developed attenuated recombinant canine distemper virus (CDV) expressing SIVmac239-Env and shown that this vector can be used safely to elicit Env-specific immune responses in ferrets and non-human primates through intranasal administration
To augment the cell surface expression of trimeric HIVEnv and increase the replicative capacity of recombinant CDV (rCDV) vectors encoding the HIV glycoprotein, we have constructed chimeric immunogens in which signal peptide (SP), transmembrane domain (TM), or cytoplasmic tail (CT) domains in HIV-Env have been replaced with analogous sequences from the vesicular stomatitis virus (VSV)-G or CDV-F glycoproteins and compared cell surface protein expression, antibody binding profiles and Env function in transient expression assays
Summary
Our aim is to develop replication-competent viral vectors for mucosal delivery, which express HIV-Env immunogens that closely mimic the trimeric glycoprotein spike found on HIV particles
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