Development of an Optical Detection-Based Universal Biochemical Blood Analysis Platform

Abstract

The development of a cost-efficient and sensitive platform for biochemical analysis of blood serum and its realization in the low resource areas is one of the imperative challenges to establish a robust healthcare ecosystem. The present work demonstrates the design of a universal platform, capable of performing all biochemical analyses of blood serum by measuring the absorbance of light through the test sample. To verify the working of the developed platform, the concentration of glucose was estimated in blood serum. The detection of glucose has been accomplished in a linear range of 1 mg/dL to 400 mg/dL with detection of limit 1 mg/dL ( <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\text{R}^{2} =0.9875$ </tex-math></inline-formula> , n = 3). The stability analysis demonstrates improved stability in the output as compared to the conventional analyzer with an average standard deviation of 0.32 calculated for n = 5. Human blood samples were tested with the developed platform and the results were in line with the pathology laboratory. The developed platform offers the advantages of automation, low cost, portability, simple instrumentation, flexibility, and an easily accessible interface. Due to the use of a huge processing capability processor, the analysis time reduces to half a minute which yields fast analysis and high throughput. The stability and accuracy also improve owing to the employment of high-resolution electronics components. Overall, the proposed framework is an attractive solution to be incorporated in the low resource area as a universal platform for all biochemistry analysis simply by varying the wavelength of light and reagent.