Abstract
Abstract An isocratic reversed-phase ion-pair high-performance liquid chromatographic method was developed for monitoring the photodegradation of ciprofloxacin in aqueous solutions. Changes in the chromatographic behaviour of ciprofloxacin and its degradation products under the influence of organic modifiers, anionic and cationic ion-pair reagents and pH were investigated. Baseline separation was achieved with use of an eluent of acetonitrile-phosphoric acid (20 mM, pH 2.3) containing 2.5 mM 1-heptanesulphonic acid sodium salt. The method was applied to the quantification of ciprofloxcin in irradiated solutions. The calibration graphs were linear from 5 to 150 μg/ml (r > 0.9999). Within the same range, the accuracy was 99–102% of the expected values. The intra-assay repeatability of the peak areas and retention times was good (R.S.D. ⩽ 039%). Likewise the intra-day reproducibility of the method was good: R.S.D. values (n = 6) were ⩽ 2.3% and 1.8% for the lowest and highest concentrations, respectively. The inter-day precision gave a mean R.S.D. of 2.4% for peak areas and R.S.D. of 3.3% for retention times during a working period of three months.
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