Abstract
A COOH-F-MWCNT-Nafion-Ru(bpy)32+-Au-ADH electrogenerated chemiluminescence (ECL) electrode using COOH-functionalized MWCNT (COOH-F-MWCNT) and Au nanoparticles synthesized by the radiation method was fabricated for ethanol sensing. A higher sensing efficiency for ethanol for the ECL biosensor prepared by PAAc-g-MWCNT was measured compared to that of the ECL biosensor prepared by PMAc-g-MWCNT, and purified MWCNT. Experimental parameters affecting ethanol detection were also examined in terms of pH and the content of PAAc-g-MWCNT in Nafion. Little interference of other compounds was observed for the assay of ethanol. Results suggest this ECL biosensor could be applied for ethanol detection in real samples.
Highlights
The detection and quantification of alcohol with high sensitivity, selectivity, and accuracy are required in many different fields from the food industry and clinic analysis, to investigation of Sensors 2009, 9 adulterations and monitoring of natural processes [1]
Au nanoparticles after the immobilization of the Ru(bpy)32+ on the surface of COOH-F-MWCNTNafion composite film, which was prepared by hand-casting of COOH-F-MWCNT-Nafion composite on the surface of the GC electrode
We performed the Radiation-induced graft polymerization (RIGP) of Acrylic acid (AAc) and methacrylic acid (MAc) on the purified MWCNTs in aqueous solution
Summary
The detection and quantification of alcohol with high sensitivity, selectivity, and accuracy are required in many different fields from the food industry and clinic analysis, to investigation of Sensors 2009, 9 adulterations and monitoring of natural processes [1]. In order to immobilize enzymes onto CNTs, CNTs must have hydrophilic properties, and not remain in its hydrophobic state, to results in interaction between enzymes and the surface of CNTs. Radiation-induced graft polymerization (RIGP) is a beneficial method for introduction of functional groups into different polymer materials using specially selected monomers. We functionalized MWCNTs by radiation-induced graft polymerization (RIGP) of vinyl monomers with carboxylic acid groups in order to immobilize Ru(bpy)32+ and improve solubility and sensitivity. We prepared the Au nanoparticles by radiation-induced reduction of Au ions in aqueous solution for immobilization of enzymes. Au nanoparticles after the immobilization of the Ru(bpy)32+ on the surface of COOH-F-MWCNTNafion composite film, which was prepared by hand-casting of COOH-F-MWCNT-Nafion composite on the surface of the GC electrode. The prepared ECL biosensor was applied for ethanol detection in Soju (a Korean distilled alcoholic beverage) and beer
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