Abstract
ObjectiveTo evaluate a novel surface plasmon resonance (SPR) system for the direct measurement of tumor marker carbohydrate antigen 15‐3 (CA15-3) in human saliva. Design and methodsWe measured the presence of the tumor marker carbohydrate antigen 15‐3 (CA15-3) in human saliva using 2 different surface plasmon resonance (SPR) systems. To compare the sensitivity of an SPR biosensor based on thin-film Au/ZnO and the Biacore SPR system, we prepared CA15-3 samples in saliva and analyzed intensity responses to the samples at various concentrations of CA15-3. ResultsThe linear detection range of CA15-3 in human saliva with the SPR system based on thin-film Au/ZnO was 2.5–20U/mL (the cut-off point in cancer patients is around 4U/mL). The linear range with the Biacore SPR system was 40–300U/mL. ConclusionsThese results show that thin-film Au/ZnO-based SPR systems have higher sensitivity and can be used for measuring the levels of CA15-3 in human saliva without concentrating the samples.
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