Abstract
BackgroundCuscuta species known as dodder, have been used in traditional medicine of eastern and southern Asian countries as liver and kidney tonic. Flavonoids are considered as the main biologically active constituents in Cuscuta plants especially in C. chinensis Lam.ObjectiveIn the present study, a fast, simple and reliable method for the simultaneous determination and quantization of C. chinensis flavonols including hyperoside, rutin, isorhamnetin and kaempferol has been developed.Materials and methodsThe chromatographic separation was carried out on a reversed phase ACE 5 C18 with eluting at a flow rate of 1 ml/min using a gradient with O-phosphoric acid 0.25% : acetonitrile for 42 min. UV spectra were collected across the range of 200–900 nm, extracting 360 nm for the chromatograms. The method was validated according to linearity, selectivity, precision, recovery, LOD and LOQ.ResultsThe method was selective for determination of rutin, hyperoside, isorhamnetin and kampferol. The calibration graphs of flavonols were linear with r2 > 0.999. RSDs% of intra- and inter-day precisions were found 1.3&3.4 for rutin, 1.5&2.8 for hyperoside, 1.3&3.3 for isorhamnetin and 1.7 & 2.9 for kaempferol which were satisfactory. LODs and LOQs were calculated as 1.73 & 8.19 for rutin, 0.09 & 4.19 for hyperoside, 2.09 & 6.3 for isorhamnetin and 0.18 & 0.56 for kaempferol. The recovery averages of above-mentioned flavonols were 90.3%, 97.4%, 98.7% and 90.0%, respectively.ConclusionThe simplicity of the method makes it highly valuable for quality control of C. chinensis according to quantization of flavonols.
Highlights
Cuscuta is a genus consisted of about 100–170 species of yellow, orange, red and rarely green parasitic plants which is the only genus in the family of Cuscutaceae
The method was selective for determination of rutin, hyperoside, isorhamnetin and kampferol
Cuscuta Semen, a crude drug prepared from the seeds of Cuscuta chinensis Lam., is commonly used in traditional medicine as a liver and kidney tonic [1]
Summary
Cuscuta is a genus consisted of about 100–170 species of yellow, orange, red and rarely green parasitic plants which is the only genus in the family of Cuscutaceae. Cuscuta Semen, a crude drug prepared from the seeds of Cuscuta chinensis Lam., is commonly used in traditional medicine as a liver and kidney tonic [1]. Many investigations have been established different biological effects of this commonly used Chinese medicine Their contents can be an important index in quality evaluation of this crude drug. Total flavonoids and total polysaccharides were determined by colorimetric method, but the results could not reflect the drug’s quality exactly and rapidly [14,15]. These methods suffered from low resolution and sensitivity. Flavonoids are considered as the main biologically active constituents in Cuscuta plants especially in C. chinensis Lam
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