Abstract
In this work, a novel dispersive liquid–liquid microextraction procedure was developed for the determination of ergosterol using high-performance liquid chromatography with photodiode array detection. The effect of several parameters, such as the selection of extraction and dispersive solvents and their volumes, extraction time, salt concentration and, pH, were studied. Under optimized experimental conditions, the calibration plot was found to be linear in the range of 0.02–10 µg mL−1, with a correlation coefficient of 0.9995. The limits of detection and limit of quantification were found to be 0.006 and 0.02 µg mL−1, respectively. The enrichment factor was 85. The average recoveries, measured at two concentration levels, were in the range of 95–101%, with RSD less than 14%. The developed method was applied for the determination of ergosterol as a biomarker of mycorrhizae presence in Norway spruce roots and various fungi samples.
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