Abstract
The aim of this study was to evaluate the diagnostic performance of a multiplex bead assay for the simultaneous detection of antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis. Sera from Eurasian wild boar of known serological status for TB (64 seropositive, 106 seronegative), Brucella (30 seropositive, 39 seronegative), and Trichinella (21 seropositive, 97 seronegative) were used for the development and evaluation of the assay. Magnetic beads coated with recombinant MPB83 antigen (TB), a whole-cell B. suis 1330 antigen, and an E/S T. spiralis antigen were used for the detection of specific antibodies using Bio-Rad Bio-Plex technology. The sensitivities (Se) and specificities (Sp) of the multiplex assay were, for M. bovis, 0.98 and 0.86; for B. suis, 1.00 and 0.97; and for T. spiralis, 0.90 and 0.99 (Se and Sp, respectively). The results show the diagnostic potential of this assay for the simultaneous detection of antibodies against M. bovis, B. suis, and T. spiralis in wild boar.
Highlights
Introduction published maps and institutional affilMycobacterium bovis, Brucella suis, and Trichinella spiralis are among the zoonotic pathogens transmitted from animals, including wildlife, to humans
The aim of the study was to develop and evaluate a multiplex bead assay for the simultaneous detection of antibodies against M. bovis, B. suis, and T. spiralis in wild boar sera
The relatively early appearance of anti-MPB83 antibodies, around 4 weeks post-infection in cattle [25], the increased Se of serology using the MPB83 antigen in experimentally infected cattle and goats [25,26], and the characterization of this antigen as one of the most immunodominant antigens most commonly recognized by antibodies from M. bovis-infected cattle, white-tailed deer, wild boar, warthogs, and badgers [27] were the reasons for the selection of this single antigen for our assay
Summary
Mycobacterium bovis, Brucella suis, and Trichinella spiralis are among the zoonotic pathogens transmitted from animals, including wildlife, to humans. Wild boar represent a major reservoir and a potential source of infection among species [1]. This significant role of wild boar has been well documented with the isolation of matching strains of M. bovis, the causative agent of bovine tuberculosis from wild boar and cattle [2,3,4]. Regarding T. spiralis, the application of eradication programs contributed to the reduction of the infection rate, notably in areas with low population density of wild boar, suggesting their key role in the maintenance iations
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