Abstract
We have developed a reversed phase high performance liquid chromatography pulsed amperometric detection (RPHPLC-PAD) method for the determination of paromomycin. It is sensitive, repeatable, and selective without the pretreatment step.Trifluoroacetic acid–water was utilized as the eluent and detected by PAD under NaOH alkaline conditions. The paromomycin detection limit (S/N=3.3) was 2μgmL−1 and the quantification limit (S/N=10) was 6μgmL−1. Coefficients of linear regression were higher than 0.99 for concentrations between 6.25 and 200μgmL−1. The intra and inter-day precision (RSD) was less than 6.5%. The average recoveries were 97.53–102.01%. The proposed HPLC-PAD method presented advantageous performance characteristics and it can be considered suitable for the evaluation of paromomycin loaded nanogel formulation in ex vivo permeation and in vitro release studies.
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