Abstract
Antibiotic residues in cosmetics can cause seriously healthy problems such as sensitization, systemic toxicity, or even death. The developments of rapid methods for multiple antibiotics detection are needed to conveniently detect out the illegal additives. In this study, a simple, cost-effective and time-saving immunoassay, multi-analyte dot enzyme-linked immunosorbent assay (Multi-Dot-ELISA), for the simultaneous detection of three most common abused antibiotics (CPFX, TC, and SMD) was successfully designed and developed. Based on the competitive Multi-Dot-ELISA, real samples from 5 of 15 (33%) commercial cosmetic products were found CPFX and SMD additives which are banned in China. We demonstrate that this rapid and low-cost platform is a promising tool in human health protection.
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