Abstract
In this study the effect of the ethanol concentration of Bougainvillea x buttiana extracts on the flavonoids content, and its antioxidant and cytoprotective activities in vitro were determined and compared. For the elucidation of the chemical constituents, the high-performance liquid chromatography method (HPLC) was used, and verification of the antioxidant activity was carried out using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical method. The cytoprotective effects of extracts were determined by exposure to hydrogen peroxide. The HPLC analysis showed the presence of rutin, quercetin-3-glucoside and quercetin rhamnoside. Among the extracts investigated the best recuperation of the rutin content was observed in extracts with 80% ethanol (83 ± 5 mg/mL). The amounts of rutin present in all extracts contribute to the antioxidant capacity and the IC50 was 427.49 (0%), 275.41 (50%), 271.61 (80%), and 272.14 (100%) µg/mL. The lowest percentage of viability was found in the cultures exposed to 100% ethanol (92%). In cultures exposed to hydrogen peroxide the percentages of protection were 25%, 33%, 78%, and 65% for cultures treated for 72 h at 0%, 50%, 80%, and 100% ethanol, respectively. The ethanolic extract of B. x buttiana was confirmed to have high rutin content with potent antioxidant activity, low cytotoxic and strong cytoprotective effects.
Highlights
Bougainvillea x buttiana (Bxb) is cultivated for ornamental and medicinal purposes in Mexico.Phytopharmacological studies have shown anti-inflammatory, anti-nocipetive, immunostimulant, and antioxidants activities [1,2,3]
To determine the antioxidant activity, the extracts prepared with different concentrations of ethanol were evaluated by the DPPH method assay, and quercetin was used as reference
The high-performance liquid chromatography (HPLC) method used was simple, precise, sensitive and accurate. This method was successfully applied for the routine evaluation in 5 different extracts of B. x buttiana that can be used for the analysis of quality control
Summary
Bougainvillea x buttiana (Bxb) is cultivated for ornamental and medicinal purposes in Mexico.Phytopharmacological studies have shown anti-inflammatory, anti-nocipetive, immunostimulant, and antioxidants activities [1,2,3]. Bougainvillea x buttiana (Bxb) is cultivated for ornamental and medicinal purposes in Mexico. The organic substances present in the plants extracts exhibited natural variations in composition because of factors such as season, geography and cultivar that can have a substantial effect on the quality and the restoration fee of herbal drug treatments [4]. Bxb is inserted in medicinal plants of Mexico, its extract has not been carried out studies for the analytical validation of its chemical constituency. Separation techniques such as high-performance liquid chromatography (HPLC) are used for validation through the ability to identify compounds in a comparison with standards of known purified substances. Loads of biochemical and pharmacological properties of plants extracts have been associated to flavonoid content [5]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
