Abstract
Initiated by the lack of oilseed rape cultivars (Brassica napus, genome AACC) resistant against Heterodera schachtii (beet cyst nematode, BCN) an introgression breeding programme has been started years ago. Oilradish genotypes (Raphanus sativus, genome RR) possessing a high degree of nematode resistance served as gene donors. As compared to all rapeseed genotypes being susceptible to cyst nematodes some highly resistant F1 hybrids (ACR), derived from the intergeneric cross B. napus x R. sativus, were detected. Allohexaploid plants (AACCRR) were produced by colchicine chromosome doubling and used in a backcross programme with summer type rapeseed as pollen donor. Selection of resistant individuals was performed by nematode infection tests in vivo. A progeny consisting of 51 BC2 plants derived from one highly resistant BC1 individual was tested for the degree of nematode resistance. Three resistant individuals were detected which are used to generate a BC3 progeny. In order to overcome the laborious testing procedure in vivo a molecular method for detecting plants containing a gene for nematode resistance would be very useful. Marker assisted selection would accelerate the process of breeding for a highly resistant substitution or translocation line. To find a molecular marker a segregating oilradish population is produced for a bulked segregant analysis. Based on putatively homologous DNA sequences of other resistance genes, primer pairs will be developed and tested to be applied as markers to accelerate selection in backcross generations without running nematode resistance tests in every generation.KeywordsSugar BeetBrassica NapusOilseed RapeBulk Segregant AnalysisHelianthus AnnuusThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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