Abstract
De novo assemblies of transcriptome sequence of F(1) interspecies hybrids between Mandarin fish Siniperca chuatsi (Basilewsky) (♀) and Golden mandarin fish Siniperca scherzeri Steindachner (♂) were generated using Illumina sequencing. In the present study, 64 microsatellite primer pairs were designed on basis of the transcriptome data. Those primer pairs were then characterized both in S. scherzeri and S. chuatsi. Thirty-eight polymorphic loci were detected in 37 individuals from a wild S. scherzeri population with 2-10 alleles per locus. The values for observed and expected heterozygosities ranged from 0.0000 to 0.8378 and from 0.4235 to 0.8771, respectively. Meanwhile, 36 microsatellite loci were polymorphic in 30 individuals from a wild S. chuatsi population with 2-8 alleles per locus. High cross-species transferability was detected. These markers will facilitate further studies on the conservation genetics, population structure and construction of high-density linkage map. What's more, they will be useful for parental selections in controlled hybridization breeding programs.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
