Abstract
We characterized nine new microsatellite markers isolated from (GT)n and (CT)n microsatellite-enriched genomic libraries of the sea squirt (Halocynthia roretzi). All markers were polymorphic in 92 individuals from a single natural population with 2–21 (mean 9.22) alleles per locus. The observed and expected heterozygosity of these markers were 0.086–0.886 and 0.102–0.870, respectively. One marker (Hr2004) significantly deviated from Hardy–Weinberg equilibrium. These new microsatellite markers should be useful for assessing the genetic diversity and population structure in H. roretzi.
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