Abstract
Although much research has been carried out on the efficacy of possible breast cancer drug treatments in two-dimensional in vitro cell cultures, these may not adequately represent the response of tumors observed in vivo. As an alternative, multicellular three-dimensional spheroids provide an in vitro microenvironment that more closely resembles tumors in vivo. The objective of this study was to develop a 3-dimensional spheroidal cell culture assay using the triple-negative human breast cancer cell line, Hs578T. The results of the project provide a better understanding of the variables of the spheroid culture technique, including cell morphology and viability. It is hoped that future experiments will provide a better understanding of the impact of one anti-proliferative chemotherapy agent, Paclitaxel, on spheroidal Hs578T cells that will, ultimately, provide a model that more closely resembles in vivo cancer growth.
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