Abstract
Reliable plant analysis is a challenging task due to the physical character and chemical complexity of plant matrices. First of all, it requires the application of a proper sample preparation procedure to fully isolate the analyzed substances from the plant matrix. The high-temperature liquid–solid extraction is commonly applied for this purpose. In the light of recently published results, however, the application of high-temperature extraction for polyphenolics analysis in plants is disputable as it causes their transformation leading to erroneous quantitative estimations of these compounds. Experiments performed on different plants show that the transformation/degradation of quercetin and its glycosides is not induced by sea sand disruption method (SSDM) and prove the method to be most appropriate for the estimation of quercetin and its derivatives in plants. What is more, the application of SSDM in plant analysis allows the researcher, to determine which quercetin derivatives are native plant components and what is their true concentration. In other word, the application of SSDM in plant analysis eliminates errors in the study of plant metabolism involving quercetin and its derivatives.
Highlights
Quercetin is one of the most widely distributed polyphenolics in plants
Reliable plant analysis is a challenging task due to the physical character and chemical complexity of plant matrices. It requires the application of a proper sample preparation procedure to fully isolate the analyzed substances from the plant matrix
The results reported in the literature [5,6,7,8] reveal that the high-temperature extraction of polyphenolics with methanol and its water mixtures, i.e. the extractants typically used for the isolation of phenolics from plants, causes the hydrolysis of glycosides and results in the formation of alcoholic derivatives of glycosides and aglycones, and in degradation of the latter
Summary
Quercetin is one of the most widely distributed polyphenolics in plants. This aglycone compound occurs in fruits, vegetables, leaves and grains, often in the form of glycoside derivatives. Reliable plant analysis is a challenging task due to the physical character and chemical complexity of plant matrices First of all, it requires the application of a proper sample preparation procedure to fully isolate the analyzed substances from the plant matrix. In the light of these findings, the application of high-temperature extraction as a sample preparation technique for polyphenolics analysis in plants is disputable and makes the results obtained for a given plant unreliable. These doubts are justified by the results presented in our earlier work [9] showing that at least 23 compounds are formed from rutin, the most abundant quercetin glycoside, during its extraction under reflux
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