Abstract
Bacillus spp are accessible and cheaper sources of producing different enzymes like L-arabinose isomerase (L-AIs). The enzyme has a crucial role in food product processing and medicine. The aim of this study was identifying the Bacillus genus with the ability of producing araA gene (encoding L-AIs). In present study, 75 soil samples from Gandom Beryan area of Kerman, Iran, were collected 50 of which were identified as Bacillus spp. via traditional and molecular methods. The PCR results of the presence of araA gene encoding L-AIs showed that 9/50 isolated carried the gene. For overproduction of the gene, cloning in Escherichia coli (E. coli) XL1-blue were performed and successful cloning confirmed by PCR, real-time PCR and clonal selection. Based on the results of this study, the Bacillus cereus of Gandom Beryan area of Kerman, Iran, showed the capacity to produce the araA gene and cloning of the gene in E. coli XL1-blue was successful. Due to L-AIs deficiency, determining such accessible and cheaper sources in producing the enzyme is important.
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