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Abstract
This study aimed to determine the optimum conditions for biofilm formation by Salmonella isolates and evaluate the effect of cinnamon essential oil nanoemulsion (CEON) against Salmonella biofilms formed under these conditions. The optimum conditions for biofilm formation by Salmonella serotype Enteritidis and Salmonella serotype Typhimurium were temperatures of 27.3 and 29.7 °C, pH levels of 6.3 and 6.8, and NaCl concentrations of 0.66 and 0.65%, respectively. CEON exhibited a significant inhibitory effect even at low concentrations, with a greater impact on the biofilm of S. Enteritidis compared to S. Typhimurium. The effectiveness of CEON in removing biofilms was increased with higher concentrations and longer contact times, with better results observed at 8 °C compared to 25 °C. In conclusion, CEON demonstrated excellent anti-biofilm activity against S. Enteritidis and S. Typhimurium biofilms, suggesting its potential use as a natural and effective disinfectant in the food industry.
Published Version
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