Abstract
A convenient and easy-moving, modified, headspace solid-phase microextraction (HS-SPME) device was developed for monitoring a living plant’s volatile organic compounds (VOCs). It consisted of a polyethylene terephthalate (PET) bottle as a sampling chamber, and certain variables were considered when using the HS-SPME device, including the material used and the fiber position, the direction of the airstream, and the distance between the sample and the fan. The results from varying those factors, generated by the orthogonal array design (OAD) method, were used to optimize the modified HS-SPME conditions. Based on the current literature regarding extracting fragrances by SPME, we selected polydimethylsiloxane/divinylbenzene (PDMS/DVB) and polydimethylsiloxane (PDMS) as the fiber materials. Using the OAD method, PDMS/DVB was found to be the better fiber material when it was parallel to the fan, and also when the airstream provided positive pressure to the sample with the fan near the sample. The device was used to sample biogenic volatile compounds emitted from fresh Nymphaea caerulea (water lily) flowers, followed by gas chromatography-mass spectrometry (GC-MS) analysis. For the method validation, under the optimum conditions, the calculated detection limit value of the model compound (butyl decanoate) was 0.14 ng on column, which was equal to 1.41 ppm for the injection. The relative standard deviations of the intra-day and inter-day precisions were 1.21% and 3.05%. Thirty-three compounds were separated and identified. The main components in the vapor phase of N. caerulea were benzyl acetate (10.4%), pentadecane (15.5%), 6,9-heptadecadiene (40.1%), and 8-heptadecene (15.3%).
Highlights
Aromatic plants have been studied in recent years due to their biological characteristics and have been used in the food, cosmetics, and pharmaceutical industries [1,2,3,4]
When a plant’s volatile organic compounds (VOCs) spread into the atmosphere, the concentration ranges from parts per trillion to parts per billion, and such a light aroma is barely noticeable to humans [7]
2-L recycled polyethylene terephthalate (PET) plastic bottle used as a chamber to facilitate the extraction of volatile components from a live water lily, which was still cultured in a water basin
Summary
Aromatic plants have been studied in recent years due to their biological characteristics and have been used in the food, cosmetics, and pharmaceutical industries [1,2,3,4]. Most aromatic plants produce volatile organic compounds (VOCs) which people find to be pleasing, so they are cultivated worldwide and used in seasonings, perfumes, ethereal oils, and so on. Most of these plants have their own peculiar characteristics, and in order to acquire their essence, researchers must isolate them in a suitable amount of time [5]. Sci. 2019, 9, 1269 which is widely cultivated in Asia and used in medical, food, cosmetics, and tea processing Among members of this family, Nymphaea caerulea Savigny is one of most cultivated water lily species in many countries [6]. When a plant’s VOCs spread into the atmosphere, the concentration ranges from parts per trillion to parts per billion, and such a light aroma is barely noticeable to humans [7]
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