Abstract
A high-performance liquid chromatographic (HPLC) method for the determination of cholecalciferol (vitamin D 3) in egg yolk is described. The egg yolk samples were purified by saponification and extraction as well as efficient solid-phase extraction and semipreparative straight-phase HPLC. Vitamin D 3 was quantitated with reverse-phase HPLC using an internal standard (vitamin D 2) method and diode array detection. The suitability of the method was tested by analyzing eggs on sale through retail outlets as well as samples obtained straight from farms. The detection and determination limits of the method were 2 and 8 ng per injection, respectively. Overall mean recovery for both vitamins was 70%. The recovery of vitamin D 3 calculated on the basis of the internal standard was 94%. The day to day repeatability of the method expressed by the coefficient of variation was 3.6%. The method introduced here is well suited for the determination of vitamin D 3 contents in egg yolks; quantification was reliable and accurate.
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