Abstract
A new microextraction technique termed reverse dispersive liquid–liquid microextraction (R-DLLME) was developed. An orthogonal array design was used to optimize ultrasonic extraction conditions. In this method, the appropriate ionic liquid was injected into the sample by pipette. The performance of R-DLLME was illustrated with the determination of ursolic acid in force loquat capsule sample by RP-LC-UV detection. The optimized method exhibits a good linearity (r = 0.9989) over the studied range (2.0–60.0 μg mL−1) for ursolic acid. The recovery was 95.9–105.5%. The R-DLLME method has the advantages of simplicity of operation, rapidity, low cost, high recovery and enrichment factor.
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