Abstract
We developed a determination method for human urinary 12-hydroxyeicosatetraenoic acid (12-HETE) using LC–MS–MS. This method, which includes simple extraction and detection in the SRM mode, allows precise and accurate determination of 12-HETE. There was a significant sex difference in urinary 12-HETE levels. Chiral analysis of 12-HETE using LC–MS–MS with column-switching technique revealed that the major enantiomer was 12( S)-HETE. Furthermore, the urinary level in patients with diabetes mellitus (DM) was analyzed. The present in vivo findings indicate that there could be difference in production of 12( S)-HETE between genders and 12( S)-HETE may play a role in the pathogenesis of DM.
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