Abstract
A quantitative method for the determination of urea in human urine was studied. An ion chromatograph (IC) with a conductivity detector was used in this method, where the chromatograph was modified by placing a cation exchanger-an immobilized urease column on the injection loop with a 6-way valve in IC. A cation exchanger was prepared by the treatment of cedar sawdust with fuming sulfuric acid (exchange capacity, 1.2 meq g-resin−1). Immobilized urease was prepared by the adsorption of urease on cedar sawdust with triethylenetetramine (activity, 3500 U g−1). The reactor with a cation exchanger-an immobilized urease column could convert urea in urine to ammonium ion (conversion efficiency, 100%) even if a sample solution was passing through column at a 9 mL min−1 rate. Simultaneously, the above-mentioned reactor could also completely hold NH4+, Na+ and K+ in urine. The calibration curves of urea were very linear over 4.0 – 20 mg L−1. The urea concentration in urine could be determined within 7 min after developing the urine sample.
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