Abstract
A new method for determining total glycoalkaloid content of potatoes (TGA) was developed by extensively modifying the comprehensive titration procedure of Fitzpatrick and Osman (1). This modified titration method eliminates the need to hydrolyze the glycoalkaloids and to purify the resulting aglycones by partitioning them into an organic solvent such as benzene or methylene chloride. Instead the glycoalkaloids are precipitated using ammonium hydroxide with the precipitate being dissolved in a tetrahydrofuran-water-acetonitrile (50:30:20) mixture. An aliquot is evaporated and quantitated using the nonaqueous titration procedure developed by Fitzpatrick and Osman (1). A comparison between this modified method and a high-performance liquid Chromatographic (HPLC) procedure was made using three commercial potato varieties and five experimental selections. Results from the two methods compared favorably.
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