Abstract
Avocados (Persea americana Mill) are fruits that originally from Mexico and Central America. It has the characteristics of green flesh on the bottom of the skin and yellowing toward the seeds with a texture that is rather soft when it is ripe. Skin color varies, some are green because of chlorophyll or black content due to anthocyanin pigments. This study aimed to determine the total levels of flavonoids in the skin of avocados that were green and black. Determination of total flavonoid levels used a UV- Vis spectrophotometer. The results showed that an analysis of water content was 5.306% for green avocado skin and 7.327% for black avocado skin. The analysis of total flavonoid levels at a wavelength of 437 nm obtained respectively, the average yield was 54,950 mg/100g for green avocado skin and 29,519 mg/100g for black avocado skin. The results of this study are expected to be able to attract the interest of the community to cultivate green and black avocado plants. especially in the area of Central Sulawesi.
Highlights
The results showed that an analysis of water content was 5.306% for green avocado skin and 7.327% for black avocado skin
Aktivitas anti bakteri biji dan kulit buah alpukat ( persea americana mill .) terhadap aerobacter aerogenes dan proteus mirabilis
Summary
Alat dan bahan Alat-alat yang digunakan pada penelitian ini yaitu pipet tetes, tabung reaksi, neraca digital, spatula, Erlenmeyer 100 mL, mikro pipet, gelas kimia 100 mL, labu ukur 50 mL, shaker, corong, kertas saring, gelas ukur 100 mL, rak tabung reaksi, oven, cawan, penjepit, wadah, kuvet, spektrofotometer UV-Vis, sendok, desikator, blender dan gunting. Bahan yang digunakan pada penelitian ini yaitu sampel ekstrak kulit buah alpukat hijau dan hitam (Persea americana Mill), etanol % , etanol %, aluminium foil, tissue, aluminium klorida (AlCl3) 10%, kalium asetat, aquades, standar kuarcetin konsesntrasi 20 ppm, 40 ppm, 60 ppm, 80 ppm, 100 ppm. Panjang gelombang maksimum tersebut yang digunakan untuk mengukur serapan dari sampel ekstrak kulit buah alpukat (persea americana Mill.). Semua filtrat disatukan dan dipekatkan dengan menggunakan rotavapor sampai tidak ada lagi cairan yang menetes sehingga diperoleh ekstrak etanol kulit buah alpukat (Persea americana Mill.). Kemudian mendiamkan selama 30 menit, lalu menyaring kembali larutan untuk memisahkan filtrat dan residu.kemudian memasukan filtrat kedalam kuvet kemudian diukur nilai serapannya menggunakan alat spektrofotometri UV-Vis dengan panjang gelombang 437 nm penentuan total flavonoid dengan metode kolorimetri
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