Abstract
The minimal effective dose of a preparation of porcine FSH was determined by the assay based on the resumption of ovarian follicular development and the increase of ovarian weight in hypophysectomized immature rats. This preparation contained about 23% of ICSH by the ventral prostate assay in hypophysectomized rats. At doses of 50100γ the minimal stimulation of follicles was detected microscopically, and the follicular growth was evident at a dose of 200γ. In this dose, the definite increase of ovarian weight was, also, observed.From these results, total dose of 200γ of the preparation of FSH was determined to be the minimal effective dose stimulating both the follicular development and the ovarian weight increase.
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