Abstract
A flow injection analytical method using a walljet-type electrochemical detector was developed for determining the content of free fatty acids. A simple flow injection system was constructed. An ethanol solution containing 3 mM 2-methyl-l,4-naphthoquinone and 38 mM LiClO4 served as the carrier solution. The flow-signal response for higher fatty acids was linear between 25 and 1500 pmol test1. The free fatty acid content in various fat and oil samples was determined by both the present and conventional titration methods. The present method was found to be not only highly sensitive with high reproducibility, but also quite simple and rapid.
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