Determination of the Compensatory Growth Response, Mineral and Antioxidant Enzyme Activities of European Sea Bass Fingerlings (Dicentrarchus labrax) in Fasting and Re-feeding

Bone morphogenetic protein 15 and growth differentiation factor 9 expression in the ovary of European sea bass ( Dicentrarchus labrax): Cellular localization, developmental profiles, and response to unilateral ovariectomy

Production and characterization of recombinantly derived peptides and antibodies for accurate determinations of somatolactin, growth hormone and insulin-like growth factor-I in European sea bass ( Dicentrarchus labrax)

ISPAD Clinical Practice Consensus Guidelines 2022: Ramadan and other religious fasting by young people with diabetes.

Growth of European sea bass ( Dicentrarchus labrax L.) under hypoxic and oscillating oxygen conditions

Nutritional innovations in superior European sea bass (Dicentrarchus labrax) genotypes: Implications on fish performance and feed utilization

Circadian feeding schedules in gilthead sea bream (Sparus aurata) and European sea bass (Dicentrarchus labrax): A comparative approach towards improving dietary fish oil utilization and n-3 LC-PUFA metabolism

The exceptionally fast growth that fish experience after periods of fasting has been called ‘compensatory growth’ or ‘catch‐up’ growth. This phenomenon, reported in a wide range of fish species, has been studied in intensive aquaculture as a means of enhancing feed conversion efficiency, but the mechanisms implicated are complex and not yet fully understood. In the present study, the authors describe the cloning and sequencing of the complete coding sequences of sea bass (Dicentrarchus labrax) insulin‐like growth factor‐I (IGF‐I) and insulin‐like growth factor‐II (IGF‐II), which are potent mitogens known to play important roles in growth and development. Sea bass IGF‐I has an open reading frame (ORF) of 561 bp that encodes a mature protein of 187 amino acids, whereas IGF‐II has an ORF of 648 bp encoding a mature protein of 216 amino acids. At the amino acid level, sea bass IGF‐I shares a 30% similarity with IGF‐II. The authors then report the pattern of IGF‐I and IGF‐II gene expression in liver and myotomal muscle in response to prolonged fasting and refeeding. Nutritional status significantly influenced IGF‐I messenger RNA copy number in both liver and muscle, inducing a down‐regulation during fasting and an up‐regulation during the recovery from fasting. The trend of IGF‐II response was similar, but different feeding regimens did not affect the amounts of transcript as sharply as in IGF‐I. Taken together these data indicate that IGF‐I and IGF‐II participate in promoting sea bass muscle compensatory growth induced by refeeding.

The aim of this study was to evaluate the effect of starvation and refeeding on the growth and food intake of gilthead seabream (Sparus aurata) and seabass (Dicentrarchus labrax) and on the growth and nitrogen uptake of glasswort (Salicornia europaea) in a polyculture aquaponic system under 12 ppt salinity for 75 days. Nine small-scale autonomous aquaponic systems were used, each containing 10 gilthead seabreams (average weight of 6.33±0.73 g and average length of 5.73±0.72 cm) and 10 seabasses (5.82±0.77 g and 6.35±0.45 cm), as well as five glasswort plants. Three fish feeding treatments were performed, a control (A), in which fish were fed daily until satiation, and two fasting treatments for 4 (B) and 7 days (C). Fish growth performance was significantly lower (p<0.05) in the C treatment for both species compared to treatments A and B. Food consumption (FC) and feed conversion ratio (FCR) were significantly higher (p<0.05) in treatment C. Glasswort growth performance was significantly higher in treatment C (p<0.05). The results showed that the 4-day food-deprived fish were similar to the control fish by achieving partial compensatory growth. The more extended fasting period (7 days) resulted in significantly lower growth performance. The lipid and nitrogen retention levels in both species were significantly lower in food-deprived fish than in the control fish both before and during compensatory growth. The results suggest that a feeding schedule involving starvation-refeeding cycles is a promising feed management option for these species in polyculture aquaponic systems. The effect of food deprivation was also significantly beneficial (p<0.05) for the growth performance of glasswort compared to the control treatment.

This preliminary study assessed genotype _ diet interaction in late growth of European sea bass (Dicentrarchus labrax) fed with either a ￠sh meal (FM)- or a ￠sh oil (FO)-based diet (M) or an all-plant-based (PB) diet. Atotal of 550 ￠sh from 224 families were reared together and tagged. DNAwas sampled and microsatellites were used to assign parentage. When ￠sh weight was 192 _ 54 g, two tanks were fed with M (FM: 100%; FO: 100%) and two others with PB (FM: 0% and FO: 0%). Body weight (BW), fork length (FL) and ￠llet lipid content (CorrFat) were analysed with a linear model and with REML methodology.We observed no signi￠cant di!erences between groups, but a slightly lower (P50.03) daily growth coe⁄cient in sea bass fed PB than in those fed M. Heritability estimates of BWdi!ered signi￠cantly from zero (PB: 0.37 _ 0.18; M: 0.47 _ 0.24). Sire _ diet interactions were signi￠cant and genetic correlations ranged between 0.51 and 0.87, showing genotype _ diet interaction for BWand CorrFat. For the ￠rst time, genetic parameters in the context of total replacement of marine ￠shery by-products were estimated in European sea bass, showing re-ranking of family performances with extremely contrasted diets.

The effect of different dietary levels of L-ascorbic acid on the growth and vitamin C concentration in the tissue of European Sea bass (Dicentrarchus labrax, L.1753) with an initial body weight of 4.6±1.1 g was studied. Four practical diets were formulated to containing 10 (AA10), 25 (AA25), 42 (AA42) and 65 (AA65) mg ascorbic acid equivalent kg-1 diet. The results of a 20 week feeding trial showed that typical vitamin C-deficiency signs and high mortality in the fish fed with the AA10 diet. The fish fed with AA42 (42 mg kg-1 AA diet) showed a significantly higher final body weight than the other diets (P less than 0.05) and the concentrations of ascorbic acid (AA) in the brain of these fish were higher than in the other experimental group (P less than 0.05). In this study, the amount of storage in the organs occurred in this order: brain, kidney, liver.

Effect of fishmeal and fish oil replacement by vegetable meals and oils on gut health of European sea bass (Dicentrarchus labrax)

Otolith growth and the value and properties of the Recent Otolith Growth Index (ROGI) were studied in sea bass (Dicentrarchus labrax L.) larvae that were reared for the first month of life with four different feeding regimes: fed, non-fed, late-feeding and late two-day fast. A marking experiment using alizarin complexone was previously carried out to validate increment deposition. Daily increment deposition was observed to take place from day two after hatching (DAH). The different feeding regimes did not significantly affect the periodicity of otolith increment deposition but did affect increment width. The ROGI was used as a tool for assessing feeding-induced differences in condition. Non-fed larvae had significantly smaller otoliths than fed larvae at the same age. In the late-feeding larvae (food available from 13 DAH), increment width increased progressively once food was supplied, and reached values similar to those for fed larvae after one week of feeding. Deprivation of food for two days in post-flexion larvae (in the fourth week of larval development) was reflected in the formation of progressively narrower increments which had still not returned to normal width two days after feeding was resumed. Our results show that the width of the outermost otolith increments reflect the past feeding history and that the ROGI can be used to distinguish well fed from suboptimally nourished larvae.

The exceptionally fast growth that fish experience after periods of fasting has been called “compensatory growth”. This phenomenon has been studied in intensive aquaculture as a means of enhancing growth rates, but the mechanisms by which food intake activates an increase in somatic growth, and especially in muscle growth, are complex and not yet fully understood. In the present paper, we describe the molecular cloning and sequencing of sea bass (Dicentrarchus labrax) myostatin (MSTN) and fibroblast growth factor 6 (FGF6), which have been shown to be major genetic determinants of skeletal muscle growth, together with insulin-like growth factor I (IGF-I) and IGF-II, which are potent mitogens known to play important roles in growth and development.We then report the pattern of expression of the four aforementioned genes, in liver and myotomal muscle in response to prolonged fasting and refeeding. Nutritional status significantly influenced the expression of IGF-I, IGF-II and MSTN, whereas the muscular FGF6 expression levels were not affected by the feeding status of the animals. Taken together these data indicate that IGF-I, IGF-II and MSTN are involved in the sea bass muscle compensatory growth induced by refeeding, whereas FGF6 probably has not a role in this phenomenon.

Effect of three feeding strategies (automatic, ad libitum demand-feeding and time-restricted demand-feeding) on feeding rhythms and growth in European sea bass ( Dicentrarchuslabrax L.)

Effect of chronic ammonia exposure on growth of European seabass ( Dicentrarchus labrax) juveniles