Abstract
Theoretical considerations on the determining the binding constants (π) of ligands to proteins were carried out. Whereas for a one-subunit protein the relationship between thermal inactivation rates and ligand concentration there is a simple linear function, for a protein with two subunits, a second-order relationship is derived. If the theory for one-subunit proteins is applied to multi-subunit proteins, the derived values of π tend to be lower than the real binding constants. A method of determining the ligand binding constant for a two-subunit protein is described.
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