Determination of Soluble Sugars in Arabidopsis thaliana Leaves by Anion Exchange Chromatography

Abstract

Determination of soluble sugars is basic for the study of carbon metabolism in plants. Soluble sugar quantitation can be achieved by enzymatic methods implying different coupled reactions. Here we describe a simple method that allows rapid determination of the most abundant soluble sugars (glucose, fructose and sucrose) in Arabidopsis leaves by anion exchange chromatography. We have applied this method to study the levels of soluble sugars during the photoperiodic transition to flowering (Ortiz-Marchena et al., 2014)., 可溶性糖的测定对于植物中碳代谢的研究是基本的。 可溶性糖定量可以通过暗示不同偶联反应的酶法实现。 在这里我们描述了一种简单的方法，可以通过阴离子交换色谱法在拟南芥叶子中快速测定最丰富的可溶性糖（葡萄糖，果糖和蔗糖）。 我们已经应用这种方法来研究在光周期过渡到开花期间可溶性糖的水平（Ortiz-Marchena等人，2014）。