Abstract
Abstract A high performance anion exchange chromatography (HPAEC) isotopic ratio mass spectrometry (IRMS) method was developed for amino sugar-specific δ 13 C analysis in plant hydrolysates. The HPAEC-IRMS method provided good validation parameters and the amino sugar concentrations were similar to those obtained by reversed phase (RP) high performance liquid chromatography (HPLC) and fluorescence (Fl) detection. The limit of quantification (LOQ) was 150 μmol l −1 . This optimised HPAEC-IRMS method opens up the possibility of a glucosamine (GlcN) specific δ 13 C analysis in plant material. Thus, it was possible to determine the δ 13 C values in newly formed fungal GlcN for the first time. The formation and turnover of saprotrophic fungi was investigated by using the improved HPAEC-IRMS method for GlcN-specific δ 13 C analysis. The cultivation of saprotrophic fungi ( Lentinula edodes and Pleurotus species) in beech wood mixed with maize or wheat straw showed the preferred formation of fungal biomass from maize-derived (80%) rather than from beech wood-derived C. The results indicate a faster formation of fungal biomass from maize than from wheat straw as co-substrate.
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