Abstract
The standard rhodanese test used for extracts from animal tissues does not work in plant homogenates due to the concomitant occurrence of both enzymatic and non-enzymatic thiocyanate production. Rhodanese activity is generally determined by the enzymatic formation of SCN − from thiosulphate and cyanide. In plant extracts, non-enzymatic formation of thiocyanate strongly affects the enzyme test. In a model system the correction factors for rhodanese tests in plant material have been worked out and a calculation method is proposed.
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